UltraClean® Microbial DNA Isolation kit专门设计用于提取1.8mL微生物纯培养高质量基因组DNA。经测试能成功提取各种微生物包括真菌孢子、酵母、格兰性阴阳性菌。20分钟内快速提取DNA;珠磨研磨机械作用力高效裂解微生物细胞,增加DNA得率。获得大分子DNA片段可达50kb,不含抑制因子可直接用于PCR、限制性内切酶消化、qPCR、测序等下游实验;不使用对人体有害的溶剂,无需酶辅助。
微生物在加热、去垢剂和机械联合作用下崩解,释放的DNA吸附到硅胶离心柱上,经过清洗洗脱重悬于DNA-free的Tris缓冲液中。推荐使用我们的涡旋仪及适配器,以达到的提取效果。
使用 UltraClean® Kit 测试过的微生物类型 |
枯草芽孢杆菌 短小芽孢杆菌 大肠杆菌 粪肠球菌 铜绿假单胞菌 藤黄微球菌 | 金黄色葡萄球菌 乳酸杆菌 黑霉菌 白色念球菌 啤酒酵母 |
详细网页说明:UltraClean® Microbial DNA Isolation kit
更多技术文章:罗氏454焦磷酸测序研究发酵食品微生物多样性
参考文献:
Rita M. Gander, Dominick Cavuoti, Adnan Alatoom, Paul Southern, Jr., Debra Grant, Kathleen Salinas, Do
nna Gaffney,Jennifer MacKenzie, Linda Byrd. Pseudo-Outbreak of Vancomycin-Resistant-Enterococcus (VRE) Colo
nization in a Neo
natal Intensive Care Unit Using Spectra VRE Surveillance Medium.
Journal of Clinical Microbiology p. 810–813 March 2013 Volume 51 Number 3 Thuy Do, To
nnie Mulli, Francis Hughes and David Beighton. me
ta-trans
criptomic analysis of three oral Veillo
nella spp. Poster: Leeds Dental Institute. FACULTY OF MEDICINE AND HEALTH L. Quigley, O. O'Sullivan, T.P. Beresford, R. Paul Ross, G.F. Fitzgerald and P.D. Cotter. A comparison of methods used to extract bacterial DNA from raw milk and raw milk cheese.
Journal of Applied Microbiology, 24 APR 2012; 113(1): 96-105. Johana Husserl, Joseph B. Hughes, and Jim C. Spain. Key Enzymes Enabling the Growth of Arthrobacter sp. Strain JBH1 with Nitroglycerin as the Sole Source of Carbon and Nitrogen.
Appl. Envir. Microbiol., May 2012; 78: 3649 – 3655. Kathryn A. Harris, Dervla T. D. Kenna, Cornelis Blauwendraat, John C. Hartley, Jane F. Turton, Paul Aurora, and Garth L. J. Dixon. Molecular Fingerprinting of Mycobacterium abscessus Strains in a Cohort of Pediatric Cystic Fibrosis Patients.
J. Clin. Microbiol., May 2012; 50: 1758 – 1761. Beare, P.A., Unsworth, N., Andoh, M., Voth, D.E., Omsland, A., Gilk, S.D., Williams, K.P., Sobral, B.W., Kupko III, J.J., Porcella, S.F., Samuel, J.E., and Heinzen, R.A. Comparative Genomics Reveal Extensive Transposon-Mediated Genomic Plasticity and Diversity amoing Potential Effector Proteins within the Genus
Coxiella.
Infection and Immunity. 77(2): 642-656 (2009). Chen, Y., Zhang, W., and Knabel, S.J. Multi-Virulence-Locus Sequence Typing Clarifies Epidemiology of Recent Listeriosis Outbreaks in the United States.
Journal of Clinical Microbiology. 43(10): 5291-5294 (2009). Dauphin, L.A., Moser, B.D., and Bowen, M.D. e
valuation of Five Commercial Nucleic Acid Extraction Kits for their Ability to Inactive
Bacillus anthracis Spores and Comparison of DNA Yields from Spores and Spiked Enviro
nmental Samples.
Journal of Microbiological Methods. 76(1): 30-37 (2009).
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